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ire 1α antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology ire 1α antibody
Ire 1α Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ire+1%CE%B1/IRE1%CE%B1+Antibody/pmc12259320-60-0-7
Average 95 stars, based on 299 article reviews

ire 1α antibody - by Bioz Stars, 2026-09

95/100 stars

Images

Membrane:

Article Title: Phenethyl isothiocyanate and irinotecan synergistically induce cell apoptosis in colon cancer HCT 116 cells in vitro.
Article Snippet: Department of Medical Laboratory Science and Biotechnology, College of Medical Technology, Chung Hwa University of Medical Technology, Tainan, Taiwan Department of Surgery, China Medical University Beigang Hospital, Beigang, Yunlin, Taiwan Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, Taiwan School of Chinese Medicine for Post-Baccalaureate, College of Medicine, I-Shou University, Kaohsiung, Taiwan Department of Chinese Medicine, E-Da Cancer Hospital, Kaohsiung, Taiwan Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan Department of Applied Chemistry, National Chi Nan University, Nantou, Taiwan Department of Neurological Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan Department of Medicinal Botanicals and Foods on Health Applications, Da-Yeh University, Changhua, Taiwan School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan Department of Physiology, School of Medicine, China Medical University, Taichung, Taiwan Department of Medical Research, China Medical University Hospital, Taichung, Taiwan Department of Biological Science and Technology, China Medical University, Taichung, Taiwan

Incubation:

Article Title: Diosgenyl Saponin Inducing Endoplasmic Reticulum Stress and Mitochondria-Mediated Apoptotic Pathways in Liver Cancer Cells
Article Snippet: Diosgenin and diosgenyl saponins as the major bioactive compounds isolated from dietary fenugreek seeds, yam roots, etc. possessed strong antitumor effects.. To understand their detailed antitumor mechanisms, a fluorophore-appended derivative of diosgenin [Glc/CNHphth-diosgenin (GND)] was synthesized, starting from diosgenin and glucosamine hydrochloride in overall yields of 7−12% over 7−10 steps.. Co-localization of GND with organelle-specific stains, transmission electron microscopy, and relative protein analyses demonstrated that GND crossed the plasma membrane through organic anion-transporting polypeptide 1B1 and distributed in the endoplasmic reticulum (ER), lysosome, and mitochondria.

Binding Assay:

Imaging:

Transfection:

Knockdown:

Article Title: STING is an essential regulator of heart inflammation and fibrosis in mice with pathological cardiac hypertrophy via endoplasmic reticulum (ER) stress.
Article Snippet: To knock down STING, the small interfering RNA (siRNA) molecule (siSTING) and the control siRNA (siCon) were synthesized by Generay Biotechnology (Shanghai, China). .. The mouse PERK siRNA (siPERK) and IRE-1α siRNA (siIRE-1α) were purchased from Santa Cruz (USA) to knock down PERK and IRE-1α, respectively. .. The cells were transfected with siSTING using the Lipofectamine Plus following the manufacturer’s protocols (Invitrogen, USA).

Staining:

Article Title: Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha
Article Snippet: Cells were analyzed on FACSCantoII (BD Biosciences) and FlowJo software (Tristar), and sorted on FACS Aria III (BD Biosciences). .. Antibodies used were CD122- PerCP-eFluor710 (TM-B1/46-1222-82/1:200), CD19-eFluor 450 (eBio1D3/48-0193-82/1:300), CD24-FITC (M1-69/11-0242-82/1:250), CD25-PE (PC61.5/12-0251-82/1:400), CD27-APC (LG.7F9/47-0271-82/1:150), CD3ε-V500 (500A2/560771/1:100/BD Biosciences), CD4-FITC/APC-eFluor780 (RM4-5/11-0042-82/17-00471-83/1:280), CD44-FITC/APC-eFluor780 (IM7/11-0441-85/25-0441-U100/1:275), CD62L-PE-Cy7 (MEL-14/60-0621-50U/1:600/TONBO), NK1.1-PE-Cy7 (PK136/25-5941-82/1:400), CD8α-V500 (53-6.7/560776/1:300/ BD Biosciences), βTCR-APC-eFluor780 (H57-597/47-5961-82/1:400), γδ ΤCR-PerCP-eFluor710 (eBioGL3/46-5711-82/1:250), IL2-PE-Cy7 (JES6-5H4/25-7021-80/0.25 μg), IL4-APC (11B11/17-7041-82/0.25 μg), IL6-FITC (MP5-20F3/561363/0.25 μg), IL13-PE-Cy7 (eBio13A//25-1733-80/0.25 μg), IFNγ-FITC (XMG1.2/11-7311-81/0.25 μg), TNFα-APC (MP6-XT22/17-7321-81/0.25 μg), T-bet-PE (eBio4B10/12-5825-82/0.25 μg), Gata-3-AlexaFluor488 (TWAJ/14-9966-82/0.25 μg), RORγt-PE (B2D/12-6981-80/0.25 μg), PLZF-AlexaFluor488 (Mags.21F7/53-9320-82/0.25 μg) (all other antibodies are from Invitrogen/eBioscience). p38α/β (A-12), JNK (D-2), β-Actin (from Santa Cruz Biotechnology, p-p38 MAPK-AlexaFluor 488 (28B10), p-JNK-AlexaFluor 647 (G9), IRE-1α (14C10/3294/1:1000), and EIF2α (9722/1ː1000) from Cell Signaling). α-GalCer-loaded CD1d tetramers (1:400) and MR1-5-OP-RU-APC (NIH/1:800). iNKT cells were stained at 4 °C using α-GalCer-loaded CD1d tetramers. ..

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Image Search Results

HCAR1 maintains endoplasmic reticulum (ER) homeostasis in the retinal pigment epithelium (RPE)/choroid complex. A: Representative Western blot analysis images of the ER stress markers binding immunoglobulin protein (BiP) and protein disulfide isomerase (PDI), from the subretina of wild-type (WT) and Hcar1 -knockout (KO) mice at PT7, PT9, PT12, and PT15. Immediately to the right is the Western blot analysis quantification for BiP and PDI. B: Representative confocal images of subretinas from PT12 pups, stained with anti-PDI (red). C: Quantification of the phosphorylated IRE-1α (P-IRE-1α) relative to the total IRE-1α signal, in the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Representative images of IRE-1α are in <xref ref-type=

Supplemental Figure 5 B. D: Quantification of the level of spliced XBP1 (XBP-1s) relative to the level of β-tubulin, in the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15; for representative image of XBP-1s, see Supplemental Figure 5 C. E: Representative Western blot analyses of phosphorylated ER stress signal protein kinase RNA-like ER kinase (PERK), from the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Immediately adjacent ( right ) is the quantification of phosphorylated PERK (P-PERK) relative to total PERK. F: Representative Western blot analysis of phosphorylated eukaryotic translation initiation factor 2α (P-eIF2α) relative to total eIF2α in subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Histogram ( right ) is the quantification of eIF2α phosphorylation Western blot analysis, presented as the ratio of the phosphorylated eIF2α signal/the total eIF2α signal. G: Representative Western blot analysis and quantifications (histogram) of activating transcription factor 4 (ATF4), from the subretina of WT and Hcar1 -KO (KO) mice at PT7, PT9, PT12, and PT15. H: Quantitative RT-PCR quantifications of ATF4 downstream target gene expression in the subretina of Hcar1 −/− and WT mice at PT12. Data are presented as means ± SEM ( A and C – H ). n = 4 ( A , B , F , G , and H ) to 6 ( C – E ) per group. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Scale bars = 50 μm ( B ). ASNS, asparagine synthetase; CHOP, C/EBP homologous protein; Hsp90, heat shock protein 90; TRIB3, tribbles homolog 3. " width="100%" height="100%">

Journal: The American Journal of Pathology

Article Title: Endoplasmic Reticulum Stress Delays Choroid Development in the HCAR1 Knockout Mouse

doi: 10.1016/j.ajpath.2024.09.002

Figure Lengend Snippet: HCAR1 maintains endoplasmic reticulum (ER) homeostasis in the retinal pigment epithelium (RPE)/choroid complex. A: Representative Western blot analysis images of the ER stress markers binding immunoglobulin protein (BiP) and protein disulfide isomerase (PDI), from the subretina of wild-type (WT) and Hcar1 -knockout (KO) mice at PT7, PT9, PT12, and PT15. Immediately to the right is the Western blot analysis quantification for BiP and PDI. B: Representative confocal images of subretinas from PT12 pups, stained with anti-PDI (red). C: Quantification of the phosphorylated IRE-1α (P-IRE-1α) relative to the total IRE-1α signal, in the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Representative images of IRE-1α are in Supplemental Figure 5 B. D: Quantification of the level of spliced XBP1 (XBP-1s) relative to the level of β-tubulin, in the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15; for representative image of XBP-1s, see Supplemental Figure 5 C. E: Representative Western blot analyses of phosphorylated ER stress signal protein kinase RNA-like ER kinase (PERK), from the subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Immediately adjacent ( right ) is the quantification of phosphorylated PERK (P-PERK) relative to total PERK. F: Representative Western blot analysis of phosphorylated eukaryotic translation initiation factor 2α (P-eIF2α) relative to total eIF2α in subretina of WT and Hcar1 −/− mice at PT7, PT9, PT12, and PT15. Histogram ( right ) is the quantification of eIF2α phosphorylation Western blot analysis, presented as the ratio of the phosphorylated eIF2α signal/the total eIF2α signal. G: Representative Western blot analysis and quantifications (histogram) of activating transcription factor 4 (ATF4), from the subretina of WT and Hcar1 -KO (KO) mice at PT7, PT9, PT12, and PT15. H: Quantitative RT-PCR quantifications of ATF4 downstream target gene expression in the subretina of Hcar1 −/− and WT mice at PT12. Data are presented as means ± SEM ( A and C – H ). n = 4 ( A , B , F , G , and H ) to 6 ( C – E ) per group. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001. Scale bars = 50 μm ( B ). ASNS, asparagine synthetase; CHOP, C/EBP homologous protein; Hsp90, heat shock protein 90; TRIB3, tribbles homolog 3.

Article Snippet: 18 , Anti-rabbit IRE-1α antibody , 3294 , 1:500 , Cell Signaling Technology (Danvers, MA).

Techniques: Western Blot, Binding Assay, Knock-Out, Staining, Phospho-proteomics, Quantitative RT-PCR, Targeted Gene Expression